Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

This Anti-ABCA1 antibody is validated for use in WB, IH for the detection of ABCA1.

Western Blotting Analysis: A representative lot of MABN893 deteted ABCA1 in lysate of WT mouse brain and demonstrated a loss of signal in lysates of ABCA1 knock out adult mouse brain (Hu et al., 2008. J Lipid Res. 2008 Feb;49(2):386-93.)
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ABCA1 in human cerebral cortex, thalamus, and kidney tissues.
Produced in collaboration with MAB Institute, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATP-binding cassette sub-family A member 1, also known as ATP-binding cassette transporter 1 (ABC-1), or ATP-binding cassette 1, and encoded by the gene name Abca1 or Abc1, is a cAMP-dependent and sulfonylurea-sensitive anion transporter. ABCA1 is a member of the ABC transporter superfamily and the ABCA family. ABCA1 is a key gatekeeper influencing intracellular cholesterol transport. ABCA1 has two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain, and may act as a multifunctional polypeptide. ABCA1 has been shown to be down-regulated by endotoxins (LPS) or cytokines (TNF and IL-1) in J774 macrophages. The down-regulation by endotoxin in macrophages is not likely to be mediated by the liver X receptor/retinoic X receptor (LXR/RXR). ABCA1 interacts with MEGF10 and is widely expressed in adult tissues, with the highest levels found in uterus and pregnant uterus. Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking.

Immunogen

Epitope: Immunogen is near C-terminal.

Linear peptide corresponding to the C-terminus of Mouse ABCA1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in Calyculin and Okadaic Acid treated NIH-3T3 cell lysate.

Western Blotting Analysis: A 1 µg/mL diultion of this antibody detected ABCA1 in 10 µg of Calyculin and Okadaic Acid treated NIH-3T3 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~254 kDa observed